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Fig. 2 | BMC Veterinary Research

Fig. 2

From: Improved detection of biomarkers in cervico-vaginal mucus (CVM) from postpartum cattle

Fig. 2

a CVM from 16 cows was processed by different protocols, from the top: modified protocol without reducing agent, standard protocol with NAC and standard protocol with DTT. Total protein was analyzed by SDS-PAGE using 4–20% gradient running gel and non-reducing loading buffer to illustrate the protein bands within the range of main inflammatory biomarkers (red box). Different amount of methanol was added. Each protein column from the SDS-PAGE gel corresponds to a bar in the BCA assay graphs. b Molecular weight of main inflammatory biomarkers implicated in uterine inflammation; interleukin 1-α, IL-1β, Tumor necrosis factor alpha (TNFα), IL-6, α-1-acid glycoprotein (AGP), IL-10, chemokine (C-X-C motif) ligand 8 (CXCL-8/IL-8) and Chemokine (C-C motif) ligand 5 (CCL-5). ND: not defined. KDa; kilodalton.*www.uniprot.org

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