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Table 5 Predicted HPAIV detection percentage at different days post infection of a broiler flock by testing three (3) dead-bird pooled swab samples of sizes 5, 6 or 11 using lateral flow immunoassay FluDETECT with varying test sensitivities (se) together with 0.3% daily mortality trigger*

From: Quantifying the effect of swab pool size on the detection of influenza A viruses in broiler chickens and its implications for surveillance

Days post flock infection 1 2 3 4 5 6 7
Flock infection prevalence (%) # 0.0 0.1 0.4 2.7 13.0 32.5 48.8
1a
 3 pools of 5: se = 71% 6 27 64 88 96 99 100
 3 pools of 6: se = 71% 5 29 65 88 96 99 100
 3 pools of 11: se = 71% 6 31 69 89 97 99 100
2b
 3 pools of 6: se = 68.1% 5 27 65 88 97 99 100
 3 pools of 11: se = 57.9% 5 26 63 85 95 99 100
  1. *All results i.e. infection prevalence and detected proportions are presented as percentages
  2. #The mean simulated flock size was estimated as 24,111 birds
  3. aScenario 1: assuming that LFI test sensitivity for HPAIV is the same across pool sizes
  4. bScenario 2: assuming that LFI test sensitivity for HPAIV is different across pool sizes