|
Cycle no.
|
Amino acid
|
pmol
|
|---|
|
1
|
ND
|
–
|
|
2
|
D
|
26.1
|
|
3
|
L
|
18.9
|
|
4
|
P
|
10.5
|
|
5
|
Q
|
15.3
|
- Purified rFeIFN-α15 was separated on a SDS-PAGE gel, and its N-terminal amino acid sequence was determined by five cycles of automated Edman degradation. The N-terminal sequences of the two main bands on SDS-PAGE were determined. Results obtained from analysis of the larger, 20-kDa band are shown; results obtained with the < 20-kDa band were identical. ND: No amino acid was detected during the first cycle of degradation, suggesting that it was cysteine
