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Table 3 List of primary antibodies used for immunohistochemistry and western blotting analysis

From: Expression of vascular endothelial growth factor (VEGF) in equine sarcoid

Antibody Manufacturer Clone Host species Antigen retriever IHC Dilution WB Dilution
vWF DAKO Polyclonal Rabbit 0.05% pepsin in 0.01 M HCl (pH 2) at 37 ° C for 15 min 1:400 1:1000
bcl-2 DAKO 124 mouse Heated Citrate buffer, ph 6.0, 30 min 1:100 1:1000
VEGF Thermoscientific JH121 mouse Heated Citrate buffer, ph 6.0, 30 min, 1:200 1:200
Ki67 Santa Cruz D-6 mouse Heated Citrate buffer, ph 6.0, 30 min, 1:100 1: 1000