Table 3 Clinical scores and results of D. nodosus culture, gelatinase test and rtPCR (aprB2/aprV2 positive, two cut offs, duplicate runs) in 318 sheep from flocks considered to be free of footrot (clinically healthy), 170 sheep from flocks considered having virulent footrot (clinically affected) and 27 sheep from a closed flock (#11) that apparently eradicated virulent footrot more than 10Â years ago. Flocks were sampled from October 2014 to August 2015
| Â | Flock ID | No. sheep tested | Results | aprB2 rtPCRd | aprV2 rtPCRd | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Clinical Scoreb | Culturec | Gelatinase testd | |||||||||||||||||||
Run 1 | Run 2 | Run 1 | Run 2 | ||||||||||||||||||
0 | 1 | 2 | 3 | 4 | 5 | Mean | Benign | Virulent | Undetermined | Cut-off | Cut-off | Cut-off | Cut-off | ||||||||
40 | 35 | 40 | 35 | 40 | 35 | 40 | 35 | ||||||||||||||
Clinically healthy | 1 | 18 | 18 | – | – | – | – | – | 0 | – | – | – | – | – | – | – | – | – | – | – | – |
2 | 18 | 18 | – | – | – | – | – | 0 | 1 | – | – | 1 | – | – | – | – | 2 | 2 | 2 | 2 | |
3 | 19 | 17 | – | 1 | – | 1 | – | 0.1 | – | – | – | – | – | – | 1 | – | – | – | – | – | |
4 | 18 | 11 | 1 | 6 | – | – | – | 0.6 | – | – | – | – | – | – | 4 | – | – | – | – | – | |
5 | 19 | 19 | – | – | – | – | – | 0 | – | – | – | – | – | – | 2 | – | – | – | – | – | |
6 | 18 | 11 | 3 | 1 | 1 | – | 2 | 0.8 | – | – | – | – | – | – | – | – | 5 | 4 | 6 | 4 | |
7 | 18 | 16 | 2 | – | – | – | – | 0.1 | – | – | – | – | – | – | – | – | – | – | – | – | |
8 | 55 | 55 | – | – | – | – | – | 0 | – | – | – | – | 5 | 1 | 1 | 1 | 3 | 2 | 4 | 2 | |
9 | 81 | 81 | – | – | – | – | – | 0 | 2 | 2 | – | – | 5 | 3 | 4 | 3 | 2 | – | 5 | – | |
10 | 54 | 51 | 3 | – | – | – | – | 0.1 | – | – | – | – | 8 | – | – | – | 2 | 1 | 2 | 1 | |
Subtotal | 318 | 297 | 9 | 8 | 1 | 1 | 2 | Â | 3 | 2 | 0 | 1 | 18 | 4 | # | 4 | 14 | 9 | 19 | 9 | |
|  | 11a | 27 | 11 | 16 | – | – | – | – | 0.6 | – | – | – | – | 16 | 10 | 20 | 13 | – | – | – | – |
Clinically affected | 12 | 26 | 14 | 12 | – | – | – | – | 0.5 | 7 | – | 5 | 2 | – | – | – | – | 26 | 25 | 26 | 26 |
13 | 10 | 1 | 3 | – | 5 | 1 | – | 2.2 | 4 | 2 | 2 | – | 5 | 3 | 3 | 3 | 9 | 6 | 9 | 6 | |
14 | 36 | 20 | 8 | 3 | – | – | 5 | 1.1 | 2 | – | 2 | – | 6 | 5 | 6 | 5 | 17 | 14 | 21 | 13 | |
15 | 10 | – | – | 1 | – | 2 | 7 | 4.5 | 2 | – | 2 | – | – | – | ND | ND | 10 | 9 | ND | ND | |
16 | 10 | – | – | 4 | 3 | 2 | 1 | 3 | 5 | – | 5 | – | – | – | ND | ND | 10 | 10 | ND | ND | |
17 | 10 | – | – | 4 | 2 | 4 | – | 1 | 10 | 4 | 6 | – | 4 | 4 | ND | ND | 6 | 6 | ND | ND | |
18 | 9 | – | 9 | – | – | – | – | 4.9 | 3 | – | 3 | – | – | – | ND | ND | 9 | 9 | ND | ND | |
19 | 10 | – | – | – | – | 1 | 9 | 2.7 | 3 | – | 3 | – | – | – | ND | ND | 9 | 9 | ND | ND | |
20 | 10 | – | – | 4 | 5 | 1 | – | 1.1 | 3 | 2 | 1 | – | 10 | 10 | ND | ND | 10 | 10 | ND | ND | |
21 | 10 | – | 9 | 1 | – | – | – | 3.5 | – | – | – | – | 2 | 2 | ND | ND | 2 | 1 | ND | ND | |
22 | 10 | – | – | 1 | 5 | 3 | 1 | 3.3 | 6 | 1 | 5 | – | – | – | ND | ND | 10 | 10 | 10 | 10 | |
23 | 10 | – | – | – | – | 10 | – | 4 | – | – | – | – | – | – | ND | ND | 10 | 10 | ND | ND | |
24 | 9 | – | – | 5 | 2 | 2 | – | 2.6 | – | – | – | – | – | – | ND | ND | 9 | 8 | ND | ND | |
Subtotal | 170 | 35 | 41 | 23 | 22 | 26 | 23 | Â | 45 | 9 | 34 | 2 | 27 | 24 | 9 | 8 | 137 | 127 | 66 | 55 | |