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Table 2 Details of the primers used for quantitative real time PCR analysis of chicken mRNAs

From: Evaluation of hepatic lipogenesis and antioxidant status of broiler chickens fed mountain celery

Target Target Primers PCR product (bp) Accession No.
β-Actin F:5’-AGCGAACGCCCCCAAAGTTCT-3’ 139 NM_205518.1
R:5’-AGCTGGGCTGTTGCCTTCACA-3’   
FAS F:5’-GCTGGCTACAGTGGTGGACT-3’ 129 NM_205155.2
R:5’-CCACCTCGAACCACCAAAGC-3’   
LPL F:5’-CTCCGATCCCGAAGCTGAGATG-3’ 106 NM_205282.1
R:5’-CTGTCCAGGAACCAGGTAGC-3’   
ACC F:5’-CAACGAGTCGGGCTACTACC-3’ 146 J03541.1
R:5’-GGTCCTTGGTCACGTATGGG-3’   
MAE F:5’-GGACCTCGAAGCCTTCATCC-3’ 77 AF408407.1
R:5’-GCCAGGTGTATGAGTCAGCC-3’   
CAT F:5′-TGGCGGTAGGAGTCTGGTCT-3′ 112 NM_001031215.1
R:5′-GTCCCGTCCGTCAGCCATTT-3′   
SOD1 F:5-CACTGCATCATTGGCCGTACCA-3′ 223 NM_205064.1
R:5-GCTTGCACACGGAAGAGCAAGT-3′   
  1. Abbreviations: FAS fatty acid synthase, LPL lipoprotein lipase, ACC acetyl-coA carboxylase, MAE malic enzyme, CAT catalase, SOD1 superoxide dismutase 1