Characterization of Staphylococcus aureus isolated from milk samples of dairy cows in small holder farms of North-Western Ethiopia

Table 1 Sequences of primer sets with their corresponding PCR protocols and product sizes

Target gene	Primer sequence (5′-3′)	PCR-protocol	Amplicon size	Reference
femA	F: TGCCTTTACAGATAGCATGCCA	1	142 bp	[52]
femA	R: AGTAAGTAAGCAAGCTGCAATGACC	1	142 bp	[52]
mecA	F: GGCTATCGTGTCACAATCGTT	2	689 bp	[53]
mecA	R: TCACCTTGTCCGTAACCTGA	2	689 bp	[53]
spa	F: AGACGATCCTTCGGTGAGC	3	Variable	[54]
spa	R: GCTTTTGCAATGTCATTTACTG	3	Variable	[54]
lukM	F: TGAGTGGGTATGGCATGAAAGA	4	572 bp	[20]
lukM	R: TGGACATTTTGTGTTACACCCC	4	572 bp	[20]
lukF’	F: ACTCAGGCTATACCAACCCA	1	425 bp	[20]
lukF’	R: CGAGCTACTCTGTCTGCCAC	1	425 bp	[20]
pvl	F: GCTGGACAAAACTTCTTGGAATAT	5	85 bp	[55]
pvl	R: GATAGGACACCAATAAATTCTGGATTG	5	85 bp	[55]
tst	F: CAACATACTAGCGAAGGAACT	6	277 bp	[56]
tst	R: GATATGTGGATCCGTCATTCA	6	277 bp	[56]

¹ 95 °C for 2 min, 35× (95 °C for 30 s, 59.5 °C for 30 s, 72 °C for 35 s), 72 °C for 5 min and 12 °C for ∞
² 95 °C for 1.5 min, 30× (95 °C for 45 s, 55 °C for 30 s, 72 °C for 45 s), 72 °C for 5 min and 12 °C for ∞
³ 95 °C for 2 min, 30× (95 °C for 45 s, 60 °C for 45 s, 72 °C for 1:30 min), 72 °C for 5 min and 12 °C for ∞
⁴ 95 °C for 2 min, 33× (95 °C for 30 s, 58.2 °C for 30 s, 72 °C for 30 s), 72 °C for 5 min and 12 °C for ∞
⁵ 95 °C for 2 min, 35× (95 °C for 30 s, 55.5 °C for 30 s, 72 °C for 35 s), 72 °C for 5 min and 12 °C for ∞
⁶ 95 °C for 2 min, 35× (95 °C for 30 s, 59 °C for 30 s, 72 °C for 30 s), 72 °C for 5 min and 12 °C for ∞

ISSN: 1746-6148