Table 1 The primers of amplification for both DEV UL1 and UL22
From: Co-localization of and interaction between duck enteritis virus glycoprotein H and L
Name of Primers | Primers | Restriction Enzyme |
|---|---|---|
UL1t-F | 5′- CCGGAATTCATGTACCCGGTTATCGAAGGAG − 3′ | EcoRI |
UL1t-R | 5′- CCCAAGCTTTCGTCGGCCATGGTGTCCGGG − 3 | HindIII |
UL1-1F | 5’-CGGAATTCATGGGTTCCAGATGGAAGGC-3′ | EcoRI |
UL1-1R | 5′- GGGGTACCCCTCGTCGGCCATGGTGTCCGG − 3′ | KpnI |
UL1-2F | 5′- CCGGAATTCATGGGTTCCAGATGGAAGGCC -3′ | EcoRI |
UL1-2R | 5’-ACGCGTCGACCTATCGTCGGCCATGGTGTCC -3′ | SalI |
UL22-1F | 5′- CGGAATTCATGTCGCAGCTTACGGTGC -3’ | EcoRI |
UL22-1R | 5′- GGGGTACCCCTTCTTCATTGCTTAACAGTTC -3’ | KpnI |
UL22-2F | 5′- CGGAATTCATGTCGCAGCTTACGGTG −3’ | EcoRI |
UL22-2R | 5′- CCCTCGAGTCATTCTTCATTGCTTAACAG − 3’ | XhoI |