Primer
|
Sequence(5′- 3′)
|
Positions
|
---|
PRV ΔTK En pa F
|
CGGTATTTACGATGCGCAGACCCGGAAGCAGAACGGCAGCGCTCACGGCCCTGCGCAACGGGATGACGACGATAAGTAGGGATAAC
|
59,655
|
PRV ΔTK En pa R
|
GTTGACCAGCATGGCGTAGACGTTGCGCAGGGCCGTGAGCGCTGCCGTTCTGCTTCCGGGGGGTAATGCCAGTGTTACAACCA
|
60,081
|
PRV ΔTK check F
|
CGGATCTACCTCGACGGCGCCTA
|
59,524
|
PRV ΔTK check R
|
TTGTACGCGCCGAAGAGGGTGT
|
/
|
PRV BAC H1 F
|
GTACCCGTACACCGAGTCGT
|
121,170
|
PRV BAC H2 R
|
TTGTGGACCCGCGCGAACAT
|
126,648
|
PRV ∆gE check F
|
AGCCCCGGGAAGATAGCCAT
|
123,141
|
PRV ∆gE check R
|
ATCGCGGAACCAGACGTCGAAG
|
125,157
|
PRV gD part F
|
GGTGCGCGCACCTGCTGTACTTTA
|
121,385
|
PRV gD part R
|
AGATGTAGACGCACACGCCCACCAG
|
122,191
|
- Positions indicate the position of the first base of 5′ terminal on the reference genome sequence NC_006151.1. “/” indicate the primer is not on the genome of PRV but the Kanamycin resistance mark gene