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Table 3 Detection limits of one-step RT-PCR and RtF-RT-LAMP for the Fukuoka-1 Tr(−) strain

From: Development of pooled testing system for porcine epidemic diarrhoea using real-time fluorescent reverse-transcription loop-mediated isothermal amplification assay

TCID50

2 × 103

2 × 102

2 × 101

2 × 100

2 × 10− 1

2 × 10− 2

2 × 10− 3

One-step RT-PCR

+

+

–

–

–

–

–

RtF-RT-LAMP

(amplification time mm:ss)

+

16:00

+

18:15

+

23:45

+

35:45

±

–

–

  1. + Positive in duplicate
  2. - Negative in duplicate
  3. ± One positive and one negative in duplicate
  4. From 2 × 103 to 2 × 10− 3: tenfold serial dilution of 2 × 104 TCID50 PEDV Fukuoka-1 Tr(−) strain
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BMC Veterinary Research

ISSN: 1746-6148

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