Fig. 4

Protein expressions of several inflammatory factors and casein synthesis in the mammary glands of dairy cows in the LC and HC groups. a shows the protein expression of several inflammatory factors in mammary gland tissue, while (b) shows expression of casein synthesis proteins in the mammary glands of dairy cows in the LC and HC groups. The samples used for western blot derived from the same experiment and the blots for target proteins were conducted in parallel. GAPDH was used as an internal reference protein, and the band density was used to quantify protein expression. The cropped bands 1–4 bands represent the LC group, while 5–8 bands represent the HC group. The significant differences are indicated (**P < 0.01)