Table 1 The primers and their applications in this study
Primer | Sequence (5` → 3`) | Application |
|---|---|---|
TLR21 F1 TLR21 R1 | CTACAGTTTCAGGAGTTGCA TACGATTGTATCGATAGCTCAG | cDNA amplification |
5′- GSP Outer 5′- GSP Inner 3′- GSP Outer 3′- GSP Inner | TGGCAAGCGAGTTTGGTAGACGGGTGGA TAGACGGGTGGAGTGATGCAGGGTG CCAGCTATCGTCTCTTTCAAGA GCAACYGTCTACTTATCACC | RACE gene specific primers |
TLR21 F2 TLR21 R2 40S F 40S R | AAGGACCAGGAGGAGAAAT AGAGCCGAAATGAAGAACC CCGTGGGTGACATCGTTACA TCAGGACATTGAACCTCACTGTCT | Real-time PCR |