S100A12 concentrations and myeloperoxidase activities are increased in the intestinal mucosa of dogs with chronic enteropathies

Hanifeh, Mohsen; Sankari, Satu; Rajamäki, Minna M.; Syrjä, Pernilla; Kilpinen, Susanne; Suchodolski, Jan S.; Heilmann, Romy M.; Guadiano, Phillip; Lidbury, Jonathan; Steiner, Jörg M.; Spillmann, Thomas

doi:10.1186/s12917-018-1441-0

Table 1 Mucosal S100A12 and MPO levels in relation to histopathologic changes in the duodenum and ileum

From: S100A12 concentrations and myeloperoxidase activities are increased in the intestinal mucosa of dogs with chronic enteropathies

Histopathological findings	n	Severity groups	S100A12 (μg/L) Median (IQR)	P value^a	MPO (ΔA/min) Median (IQR)	P value^a
Duodenum	35
Total histopathological severity	35	Insignificant (n = 16)	33.65 (7.41-155.9)	0.2240	0.9 (0.2-2.95)	0.006 ^b
		Mild (n = 15)	53.12 (10.07-164.85)		1.49 (0.14-4.55)
		Moderate (n = 4)	52.22 (42-179.6)		7.54 (0.94-17.16)
Morphological criteria	35
Villus stunting	35	Normal (n = 20)	44.18 (7.41-155.9)	0.4090	1.23 (0.14-4.55)	0.1439
		Mild (n = 10)	45.02 (19.51-179.6)		1.21 (0.37-3.88)
		Moderate (n = 3)	56.27 (42.72-164.85)		2.16 (1.63-11.62)
		Severe (n = 2)	26.04 (10.07-42)		8.85 (0.54-17.16)
Epithelial injury	35	Normal (n = 30)	48.68 (7.41-164.85)	0.9122	1.23 (0.14-11.62)	0.0455 ^b
		Mild (n = 3)	28.94 (19.51-179.6)		1.58 (0.94-2.9)
		Moderate (n = 2)	34.38 (26.76-42)		9.82 (2.48-17.16)
Crypt distension	35	Normal (n = 29)	42.96 (7.41-164.85)	0.4156	1.29 (0.14-17.16)	0.0694
		Mild (n = 5)	67.87 (19.75-179.6)		0.94 (0.63-1.63)
		Moderate (n = 1)	42.72		11.62
Lacteal dilation	35	Normal (n = 19)	28.94 (7.41-155.9)	0.0889	1.24 (0.2-17.16)	0.0708
		Mild (n = 10)	54.7 (12.49-144.29)		1.07 (0.14-2.48)
		Moderate (n = 6)	53.07 (42.72-179.6)		2.81 (0.94-11.62)
Mucosal fibrosis	35	Normal (n = 31)	42.96 (7.41-179.6)	0.9191	1.36 (0.14-17.16)	0.6993
Mucosal fibrosis	35	Mild (n = 4)	63.88 (10.07-93.02)	0.9191	0.83 (0.54-3.88)	0.6993
Inflammatory criteria	35
Intraepithelial lymphocytes	35	Normal (n = 23)	42.96 (7.41-164.85)	0.9381	1.24 (0.14-17.16)	0.7695
		Mild (n = 10)	36.44 (11.53-179.6)		1.12 (0.37-4.55)
		Moderate (n = 2)	53.07 (44.42-61.71)		2.41 (1.36-3.46)
Lamina propria LPC	35	Normal (n = 2)	99.19 (42.48-155.9)	0.1963	0.53 (0.2-0.87)	0.0975
		Mild (n = 15)	24.83 (7.41-164.85)		1.24 (0.36-2.95)
		Moderate (n = 15)	50.94 (10.07-179.6)		1.36 (0.14-11.62)
		Severe (n = 3)	61.71 (42-93.02)		3.46 (0.77-17.16)
Lamina propria eosinophils	35	Normal (n = 22)	58.99 (10.07-179.6)	0.0516	0.92 (0.14-3.46)	0.0004 ^b
		Mild (n = 5)	19.75 (7.41-42.96)		0.91 (0.64-2.95)
		Moderate (n = 8)	43.33 (26.76-78.03)		3.39 (1.22-17.16)
Lamina propria neutrophils	35	Normal (n = 20) Mild (n = 7) Moderate (n = 6) Severe (n = 2)	42.72 (7.41-102.46) 61.1 (19.51-155.9) 47.92 (24.83-179.6) 113.28 (61.71-164.85)	0.1542	1.07 (0.36-17.16) 1.49 (0.2-3.88) 1.4 (0.14-11.62) 2.81 (2.16-3.46)	0.7326
Lamina propria macrophage	35	Normal (n = 32)	42.84 (7.41-164.85)	0.0439 ^b	1.39 (0.14-17.16)	0.1786
Lamina propria macrophage	35	Mild (n = 3)	155.9 (44.2-179.6)	0.0439 ^b	0.94 (0.2-1.36)	0.1786
Ileum	12
Total histopathological severity	12	Insignificant (n = 8)	106.93 (9.59-317.21)	0.9816	1.91 (0.34-2.83)	0.3260
Total histopathological severity	12	Mild (n = 4)	125.92 (16.36-151.79)	0.9816	2.3 (0.79-7.01)	0.3260
Morphological criteria	12
Villus stunting	12	Normal (n = 7)	120.84 (9.59-317.21)	0.7443	2.04 (0.34-2.83)	0.3447
Villus stunting	12	Mild (n = 5)	116.24 (16.36-151.79)	0.7443	1.77 (0.69-7.01)	0.3447
Epithelial injury	12	Normal (n = 10)	118.54 (9.59-317.21)	0.4956	1.91 (0.34-3.23)	0.2387
Epithelial injury	12	Moderate (n = 2)	84.07 (16.36-151.79)	0.4956	4.18 (1.36-7.01)	0.2387
Crypt distension	12	Normal (n = 11)	116.24 (9.59-317.21)	0.6913	2.04 (0.34-7.01)	0.4692
Crypt distension	12	Moderate (n = 1)	120.84	0.6913	0.79	0.4692
Lacteal dilation	12	Normal (n = 8)	106.93 (9.59-317.21)	0.9881	1.56 (0.34-2.83)	0.2034
Lacteal dilation	12	Mild (n = 4)	125.92 (16.36-162.43)	0.9881	2.63 (0.79-7.01)	0.2034
Mucosal fibrosis	12	Normal (n = 12)	118.54 (9.59-317.21)	–	1.91 (0.34-7.01)	–
Inflammatory criteria	12
Intraepithelial lymphocytes	12	Normal (n = 6)	109.23 (9.59-162.43)	0.6809	1.42 (0.34-2.81)	0.1787
Intraepithelial lymphocytes	12	Mild (n = 6)	123.62 (16.36-317.21)	0.6809	2.3 (0.69-7.01)	0.1787
Lamina propria LPC	12	Normal (n = 4)	118.78 (47.08-162.43)	0.5561	2.12 (0.44-2.81)	0.9999
		Mild (n = 4)	68.6 (9.59-317.21)		1.81 (0.34-7.01)
		Moderate (n = 4)	123.62 (47.32-151.79)		1.56 (0.69-3.23)
Lamina propria eosinophils	12	Normal (n = 9)	130.99 (9.59-317.21)	0.2514	1.77 (0.34-3.23)	0.2019
		Mild (n = 2)	72.35 (47.08-97.62)		1.63 (0.44-2.81)
		Moderate (n = 1)	16.36		7.01
Lamina propria neutrophils	12	Normal (n = 9)	116.24 (9.59-317.21)	0.7430	2.04 (0.34-3.23)	0.5989
Lamina propria neutrophils	12	Mild (n = 3)	120.84 (16.36-151.79)	0.7430	1.36 (0.79-7.01)	0.5989
Lamina propria macrophage	12	Normal (n = 12)	118.54 (9.59-317.21)	–	1.91 (0.34-7.01)	–

Association between mucosal S100A12 concentrations and MPO activities with severity of histopathological findings in the duodenum and ileum of dogs with CE. Statistical differences were calculated based on the log-transformed values of S100A12 and MPO. Untransformed values are reported as median (IQR)
n sample size, IQR interquartile range, LPC lymphocytes/plasma cells
^aANOVA test after logarithmic transformation of original values
^bstatistically significant association

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