Tick- and fly-borne bacteria in ungulates: the prevalence of Anaplasma phagocytophilum, haemoplasmas and rickettsiae in water buffalo and deer species in Central Europe, Hungary

Table 2 Results of molecular analyses of DNA samples from water buffalos and large game animal species

Species (body weight range)^a	Sample type	PCR positives/all tested (percentage)
		Anaplasma phagocytophilum	haemotropic Mycoplasma sp.			Rickettsia helvetica [other rickettsiae]
		Anaplasma phagocytophilum	M. wenyonii [M. ovis]	C. M. haemobos	M. suis	Rickettsia helvetica [other rickettsiae]
Water buffalo (300–500 kg)	blood	0/60	55/60 (91.2%)^{c, B}	44/60 (73.3%)^{c, A}	–	0/60
Red deer (100–200 kg)	blood	47/48 (97.9%)^c	31/48 (64.6%)^b	22/48 (45.8%)^b	–	0/48
Red deer (100–200 kg)	spleen	95/96 (99%)	24/96 (25%)^B	4/96 (4.2%)^A	–	0/96
Fallow deer (50–100 kg)	blood	24/33 (72.7%)^b	10/33 (30.3%)^a	3/33 (9.1%)^a	–	0/33
Fallow deer (50–100 kg)	spleen	81/85 (95.3%)	6/85 (7.1%)	10/85 (11.8%)	–	1/85 (1.2%) [1/85]
Roe deer (25 kg)	blood	39/65 (60%)^b	13/65 (20%)^{a, B}	1/65 (1.5%)^{a, A}	–	0/65
Roe deer (25 kg)	spleen	3/6 (50%)	3/6 (50%)	0/6	–	0/6
Mouflon	blood	1/16 (6.3%)^a	[1/16 (6.3%)]	0/16	–	1/16 (6.3%)
Mouflon	spleen	4/4 (100%)	[1/4 (25%)]	0/4	–	0/4
Wild boar	blood	6/17 (35.3%	–	–	8/17 (47.1%)	0/17
Wild boar	spleen	31/79 (39.2%)	–	–	23/79 (29.1%)	0/79

^aEstimated body weight range characteristic of the species (i.e. minimum to maximum acc. to females and males) based on local hunting bags (provided by Prof. L. Sugár)
Significance: (1) blood sample-based prevalences, which are significantly different between host species, are marked with different superscript lower case letter within columns, increasing from “a” to “c”; (2) the prevalence of M. wenyonii is marked with superscript capital letter “B”, if significantly higher than that of C. M. haemobos marked with superscript capital letter “A” in the same row; (3) arrows in circle with inverse color point to the significantly higher prevalence, when comparing the two kinds of sampled tissues (blood vs. spleen) for the same host and pathogen

ISSN: 1746-6148