ELISA | Optical density (OD) measured at | Calculation used | Positive results | Correlation between ELISA value and antibody level |
---|---|---|---|---|
BRSV | 450 nm | PP = SampleCOD /PCOD X100 | PP ≥ 10 | Positive |
BPI3V | 450 nm | PP ≥ 10 | Positive | |
BCoV | 450 nm | PP ≥ 10 | Positive | |
BHV-1 gE | 650 nm | S/N ratio = SampleOD/N2 | S/N ≤ 0.6 | Negative |
BHV-4 | 450 nm | S/P ratio = (SampleOD – N1)/(P1-N1) | S/P ≥ 0.3 | Positive |
BVD | 450 nm | S/P ratio = (SampleOD – N2)/(P2-N2) | S/P ≥ 0.3 | Positive |
H. somni | 450 nm | S/P ratio = (SampleOD – N3)/(P3-N3) | S/P > 0.6 | Positive |