Fig. 5
Sensitivity of the RealAmp and PCR. a Amplified pMD19T-fiber plasmid DNA by RealAmp (10− 1 to 10− 6); Reaction 1 2.6 ng; Reaction 2260 pg; Reaction 3 26 pg; Reaction 4 2.6 pg; Reaction 5260 fg; Reaction 6 26 fg; Reaction 7 positive (EDSV DNA) and Reaction 8 negative control (ddH2O). b 1.5% agarose gel electrophoresis result of RealAmp amplicon. c Determination of the detection limit of the PCR with RealAmp outer primers F3 and B3 (Table 1). PCR products were separated in 1.5% agarose gel. Lane 1 2.6 ng; lane 2260 pg; lane 3 26 pg; lane 4 2.6 pg; lane 5260 fg; lane 6 26 fg; lane 7 positive control; lane 8 negative control and lane M Trans2K plus DNA marker