| Primer name | Primer sequence | Nucleotide position | Fragment amplified |
---|---|---|---|---|
Real Time-PCR | A-for | 5’-AGCTACTATTATGAGACCAGCTGAG-3′ | 3767–3791 | A-B: 99 bp |
B-rev | 5′- CCTGCTGCAATAGGTGTTTTAA-3’ | 3844–3865 | ||
Hemi-nested-PCR | C-for (P3b) | 5’-CCATTTCTAAATTCTTTG −3’ | 3651–3668 | C-D: 881 bp |
D-rev (VPreva) | 5’-TTTCTAGGTGCTAGTTGAG −3’ | 4513–4531 | ||
E-rev (P4b) | 5’-AAGTCAGTATCAAATTCTT −3’ | 4201–4219 | C-E: 569 bp |