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Table 2 Primers used

From: Molecular analysis of carnivore Protoparvovirus detected in white blood cells of naturally infected cats

  Primer name Primer sequence Nucleotide position Fragment amplified
Real Time-PCR A-for 5’-AGCTACTATTATGAGACCAGCTGAG-3′ 3767–3791 A-B: 99 bp
B-rev 5′- CCTGCTGCAATAGGTGTTTTAA-3’ 3844–3865
Hemi-nested-PCR C-for (P3b) 5’-CCATTTCTAAATTCTTTG −3’ 3651–3668 C-D: 881 bp
D-rev (VPreva) 5’-TTTCTAGGTGCTAGTTGAG −3’ 4513–4531
E-rev (P4b) 5’-AAGTCAGTATCAAATTCTT −3’ 4201–4219 C-E: 569 bp
  1. The primer positions refer to the nucleotide sequence of FPV reference strain CU-4 (GenBank accession number M38246). [41]a, [42]b