Skip to main content

Table 1 Subject and procedures for collection of olfactory mucosa

From: Isolation and characterization of olfactory ecto-mesenchymal stem cells from eight mammalian genera

Genus (species) Gender & population Strain & Origin Age Anaesthesia & analgesia procedure Collection method
Mouse (Mus musculus) Male, 6a C57Bl/6NCrl, Charles River Laboratories 3 months Euthanasia; sodium pentobarbital (Ceva Santé Animale, 200 mg/kg, i.p.) Muzzle dissection
Rat (Rattus norvegicus) Male, 6a Fischer 344, Charles River Laboratories 6 weeks Sodium pentobarbital (Nembutal, 60 mg/kg, i.p.) and Buprenorphine (0.02 mg/kg i.m.) Nasal bone trepanation
Rabbit (Oryctolagus cuniculus) Male, 3
Breed, IRSEA 11 (± 1.5) months Medetomidine (Domitor®, 0.4 mg/kg i.m.), Ketamine 100 (Imalgene®, 30 mg/kg i.m.) and Buprenorphine (0.02 mg/kg i.m.) Nasal cavity exploration
Dog (Canis lupus) Male, 4 Female, 2 Multiple, pet dogsb 15 (± 1) years Zoletil (Tiletamine and Zolazepam,Virbac, 5 mg/kg i.v.) and Boutorfanole (Dolorex®, 0.1 mg/kg i.v.) Nasal cavity exploration
Horse (Equus caballus) Male, 4 Multiple, client-ownedb 18 (± 3) years Détomidine (Domosedan®, 10–20 μg/kg i.v.) Nasal cavity exploration
Sheep (Ovis aries) Female, 3 Merinos, IRSEA 7 (± 4) years Xylazine (Rompun® 2%, 0.04 mg/kg i.v.) Nasal cavity exploration
Gray mouse Lemur (Microcebus murinus) Male, 1 Female, 1 CNRS Primatology centre 7 years Diazepam (Valium, 20 mg/kg i.m.) and Ketamine (Imalgene, 100 mg/kg i.p.) Nasal bone trepanation
Macaque (Macaca fascicularis) Male, 1 CNRS Primatology centre 13 years Zoletil (Tiletamine and Zolazepam,Virbac, 5 mg/kg i.m.); then isoflurane (Belamont) Nasal cavity exploration
  1. amucosa from different individuals were pooled
  2. bclient-owned animals from veterinary clinics biopsied with owner consent