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Table 2 Enrichment of phage peptides from library by BEFV G1 protein biopanning

From: Biopanning of polypeptides binding to bovine ephemeral fever virus G1 protein from phage display peptide library

Item First round biopanning Second round biopanning Third round biopanning Fourth round biopanning
G1(μg) 10 7.5 5 2.5
Concentration of Tween20 (v/v) 0.1% 0.25% 0.5% 0.5%
Input/PFU (pfu/ml) 1 × 1011 1 × 1011 1 × 1011 1 × 1011
Output/PFU (pfu/ml) 3.7 × 106 5.2 × 107 8.7 × 107 7.2 × 108
Recovery
(Output/input)
3.7 × 10−5 5.2 × 10−4 8.7 × 10−4 7.2 × 10−3