Fig. 5

Antiviral activities of synthetic polypeptides on BEFV infection detected by real-time RT-PCR. Synthetic polypeptides T18, T25 and T18 + T25 were incubated with 100TCID₅₀/0.1 mL, respectively. The SYBR Green-based quantitative real-time PCR was used to determine BEFV RNA with specific primers for N gene. The relative expression of BEFV was normalized to GAPDH expression and analysed by the 2(^−△△Ct) method. Each treated sample was run in triplicate. Statistical analysis was performed by one-way analysis of variance and values were considered significant when p < 0.05. Figures were performed using the GraphPad™ Prism 5.0 software