Fig. 2

Phenotype of cattle MoDCs differentiated in the presence or absence of 1,25 (OH)₂D₃. Phenotype of MoDCs or 1,25 (OH)₂D₃ conditioned MoDCS derived from cattle CD14 + ve monocytes was analysed by flow cytometry. MoDCs were single stained with primary mAbs to CD1b, CD80, CD86 or MHC-II DR and then stained with Alexa Fluor 647 IgG secondary antibody. Live, single gated cells were assessed for expression of these markers. a Data shown are the average ± the SE of the corrected median fluorescence intensity (MFI) for MoDCs (black bars) and 1,25 (OH)₂D₃ conditioned MoDCS (white bars). b Histogram for a representative animal showing the level of uptake of expression of chosen markers for MoDCs (black histograms) or 1,25 (OH)₂D₃ conditioned MoDCS (grey histograms). *denotes statistical significance for p < 0.05