Table 2 Summary of PEDV RT-qPCR results for the pre-treatment and post-treatment swabs
Treatment group | Pre-treatment Ct Value and (genomic copies/mL) | Percentage positive or suspect for PEDV | Post-treatment Ct Value and (genomic copies/mL) | Percentage positive or suspect for PEDV |
|---|---|---|---|---|
(A) Neg Control | >35 (0) >35 (0) >35 (0) >35 (0) | 0% (0 of 4) | >35 (0) >35 (0) >35 (0) >35 (0) | 0% (0 of 4) |
(B) Pos Control | 18.2 (108.56) 19.0 (108.32) 19.5 (108.17) 19.2 (108.26) | 100% (4 of 4) | 18.6 (108.44) 17.1 (108.88) 18.8 (108.38) 18.8 (108.38) | 100% (4 of 4) |
(C) Light, 1:32, 40 mins | 17.2 (108.85) 17.4 (108.79) 17.9 (108.64) 17.6 (108.73) | 100% (4 of 4) | 27.1 (105.94) 21.0 (107.73) 29.1 (105.35) 28.6 (105.49) | 100% (4 of 4) |
(D) Heavy, 1:32, 40 mins | 16.8 (108.97) 18.0 (108.61) 17.8 (108.67) 18.1 (108.58) | 100% (4 of 4) | 24.6 (106.67) 19.2 (108.26) 24.0 (106.85) 20.6 (107.85) | 100% (4 of 4) |
(E) Light, 1:16, 40 mins | 18.7 (108.41) 17.9 (108.64) 18.2 (108.56) 18.7 (108.41) | 100% (4 of 4) | 33.6 (104.02) 31.1 (104.76) 28.9 (105.41) 30.7 (104.88) | 100% (4 of 4) |
(F) Heavy, 1:16, 40 mins | 17.2 (108.85) 17.8 (108.67) 18.5 (108.47) 18.2 (108.56) | 100% (4 of 4) | 26.1 (106.23) 23.0 (107.14) 23.9 (106.88) 23.0 (107.14) | 100% (4 of 4) |
(G) Light, 1:32, 60 mins’ | 18.0 (108.61) 17.6 (108.73) 17.0 (108.91) 18.2 (108.56) | 100% (4 of 4) | 27.5 (105.82) 26.1 (106.23) 23.6 (106.97) 27.3 (105.88) | 100% (4 of 4) |
(H) Heavy, 1:32, 60 mins | 17.6 (108.73) 17.9 (108.64) 18.7 (108.41) 17.0 (108.91) | 100% (4 of 4) | 26.1 (106.23) 23.7 (106.94) 21.5 (107.59) 24.8 (106.61) | 100% (4 of 4) |
(I) Light, 1:16, 60 mins | 17.6 (108.73) 17.5 (108.76) 17.8 (108.67) 17.9 (108.64) | 100% (4 of 4) | 34.5 (103.76) 33.4 (104.08) 34.7 (103.70) 32.3 (104.40) | 100% (4 of 4) |
(J) Heavy, 1:16, 60 mins | 16.5 (109.06) 18.0 (108.61) 17.3 (108.82) 20.1 (108.00) | 100% (4 of 4) | 28.0 (105.67) 24.9 (106.58) 24.7 (106.64) 24.9 (106.58) | 100% (4 of 4) |