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Table 1 Effect of L-cysteine alone on mean integral tension (Emax (g), tonic and phasic contractions (g), amplitude (g), frequency (BPM) and effect on MIT (g) and in the presence of AOAA (100 μM) and PAG (100 μM) in myometrial strips of non-pregnant buffaloes

From: The functional and molecular studies on involvement of hydrogen sulphide in myometrial activity of non-pregnant buffaloes (Bubalus bubalis)

Myometrial activity markers

Treatment

No. of tissues (n)

pD2

E(max) / R (max) (g)

Mean Integral Tension (MIT)

L-cysteine

14

4.70 ± 0.28

0.73 ± 0.17

Phasic contraction

L-Cysteine

6

4.51 ± 0.20

0.11 ± 0.15*

Tonic contraction

L-Cysteine

6

4.63 ± 0.55

0.91 ± 0.22

Amplitude

L-Cysteine

5

7.09 ± 1.75

0.33 ± 0.06

Frequency (BPM) before adding L-cysteine

L-Cysteine

9

……

0.80 ± 0.09

Frequency (BPM) after adding L-cysteine

L-Cysteine

9

3.42 ± 0.83

0.51 ± 0.13

MIT

AOAA (100 μM) + L-Cysteine

6

3.64 ± 0.163

−1.54 ± 0.26$

MIT

PAG (100 μM) + L-Cysteine

5

1.37 ± 2.88

−0.67 ± 0.11$#

  1. Mean values from two different groups were compared using student’s ‘t’ test
  2. *P < 0.05 Tonic contractions versus phasic contractions
  3. $P < 0.05 vs. MIT of L-cysteine, #P < 0.05 vs. MIT of AOAA + L-cysteine