Fig. 4

Replication of PEDV 8aa (P70) by the addition of trypsin at various time points determined by Western blot analysis or IFA. Confluent Vero cells were inoculated with PEDV 8aa (10 MOI) and mock-medium or trypsin (1 μg/ml) was added at different time points of 0, 30 min, 1, 2, or 4 h after virus inoculation. a Cell lysates were prepared at 24 h post inoculation for Western blot analysis with antibody against PEDV or β-actin. b Cells were fixed at 24 h post inoculation for IFA staining. Panel 1 shows the cells with mock-medium treatment. Panels 2–4 show the virus-infected cells where trypsin was added at 0, 1 or 4 h post virus inoculation, respectively