The response of canine faecal microbiota to increased dietary protein is influenced by body condition

BMC Veterinary Research

Table 1 Primer set used in the present study

Target	Primers (5’➔3′)	References
Bacteria V3 region	PRBA338f ACTCCTACGGGAGGCAGCAG	[56]
Bacteria V3 region	PRUN518r ATTACCGCGGCTGCTGG	[56]
Total Bacteria	fwd CGGYCCAGACTCCTACGGG	[57]
Total Bacteria	rev TTACCGCGGCTGCTGGCA	[57]
Firmicutes	fwd GGAGYATGTGGTTTAATTCGAAGCA	[58]
Firmicutes	rev AGCTGACGACAACCATGCAC	[58]
Enterobacteriaceae	fwd CATTGACGTTACCCGCAGAAGAAGC	[59]
Enterobacteriaceae	rev CTCTACGAGACTCAAGCTTGC	[59]
Bacteriodetes	fwd GGARCATGTGGTTTAATTCGATGAT	[58]
Bacteriodetes	rev AGCTGACGACAACCATGCAG	[58]
Lactobacillus	fwd GGAATCTTCCACAATGGACG	[60]
Lactobacillus	rev CGCTTTACGCCCAATAAATCCGG	[60]
Clostridial cluster I	fwd TACCHRAGGAGGAAGCCAC	[61]
Clostridial cluster I	rev GTTCTTCCTAATCTCTACGCAT	[61]
Clostridial cluster IV	fwd ATGCAAGTCGAGCGA(G/T)G	[62]
Clostridial cluster IV	rev TATGCGGTATTAATCT(C/T)CCTTT	[62]
Clostridial cluster XIVa	fwd CGGTACCTGACTAAGAAG	[63]
Clostridial cluster XIVa	rev AGTTT(C/T)ATTCTTGCGAAC	[63]
Butyryl-CoA acetate-CoA transferase	fwd AAGGATCTCGGIRTICAYWSIGARATG)	[64]
Butyryl-CoA acetate-CoA transferase	rev GAGGTCGTCICKRAAITYIGGRTGNGC	[64]
Butyrate kinase	fwd TGCTGTWGTTGGWAGAGGYGGA;	[65]
Butyrate kinase	rev GCAACIGCYTTTTGATTTAATGCATGG	[65]

ISSN: 1746-6148