Prevalence and molecular characterization of Staphylococcus aureus isolated from goats in Chongqing, China

BMC Veterinary Research

Table 1 Primers used for nuc, mecA and virulence genes amplification

Gene Primer	Primer sequence (5′to 3′)	Amplification size (bp)	Reference
nuc	SF:GCGATTGATGGTGATACGGTT	279	[13]
nuc	SR:AGCCAAGCCTTGACGAACTAAAGC	279	[13]
mecA	SF: AAAATCGATGGTAAAGGTTGGC	533	[15]
mecA	SR: AGTTCTGCAGTACCGGATTTGC	533	[15]
sea	SF: GGTTATCAATGTGCGGGTGG	102	[16]
sea	SR: CGGCACTTTTTTCTCTTCGG	102	[16]
seb	SF: GTATGGTGGTGTAACTGAGC	164	[16]
seb	SR: CCAAATAGTGACGAGTTAGG	164	[16]
sec	SF: AGATGAAGTAGTTGATGTGTATGG	451	[16]
sec	SR: CACACTTTTAGAATCAACCG	451	[16]
sed	SF:CCAATAATAGGAGAAAATAAAAG	278	[16]
sed	SR: ATTGGTATTTTTTTTCGTTC	278	[16]
see	SF:AGGTTTTTTCACAGGTCATCC	209	[16]
see	SR: CTTTTTTTTCTTCGGTCAATC	209	[16]
seg	SF: TGCTATCGACACACTACAACC	704	[17]
seg	SR:CCAGATTCAAATGCAGAACC	704	[17]
seh	SF:CGAAAGCAGAAGATTTACACG	495	[17]
seh	SR: GACCTTTACTTATTTCGCTGTC	495	[17]
sei	SF:GACAACAAAACTGTCGAAACTG	630	[17]
sei	SR: CCATATTCTTTGCCTTTACCAG	630	[17]
sej	SF: CATCAGAACTGTTGTTCCGCTAG	142	[18]
sej	SR: CTGAATTTTACCATCAAAGGTAC	142	[18]
tst	SF: ACCCCTGTTCCCTTATCATC	326	[16]
tst	SR: TTTTCAGTATTTGTAACGCC	326	[16]
eta	SF: ATATCAACGTGAGGGCTCTAGTAC	1155	[20]
eta	SR: ATGCAGTCAGCTTCTTACTGCTA	1155	[20]
etb	SF: CACACATTACGGATAATGCAAG	604	[20]
etb	SR: TCAACCGAATAGAGTGAACTTATCT	604	[20]
pvl	SF: GTGCCAGACAATGAATTACCC	255	[19]
pvl	SR: TTCATGAGTTTTCCAGTTCACTTC	255	[19]

ISSN: 1746-6148