Fig. 2

Identifying target genes of miR-139 via the dual luciferase reporter assay. a Bta-miR-139 binding sites in the 3′-UTR of GHR. Ten nucleotides (underlined) were mutated in luciferase reporter plasmids carrying GHR 3′-UTR. b Bta-miR-139 binding sites in the 3′-UTR of IGF1R. Eight nucleotides (underlined) were mutated in the luciferase reporter plasmids carrying IGF1R 3′-UTR. c Luciferase activity of reporter plasmids carrying wild-type or mutant GHR 3′-UTR in HeLa cells, in response to co-transfection with miR-139. d Luciferase activity of reporter plasmids carrying wild-type or mutant IGF1R 3′-UTR in HeLa cells, in response to co-transfection with miR-139. Results are shown as means ± SEM from three independent experiments. Statistical analysis was conducted using one-way ANOVA.“*”, statistical significance was considered at P < 0.05