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Table 6 Primers used in qPCR analysis to determine the microbiota in fecal swabs taken 5 days post-infection with E. coli K88. The efficiency of the qPCR was ≥95% for all primers [22]

From: Molecules produced by probiotics prevent enteric colibacillosis in pigs

Phylum Class/Family/Genus Species /Group Primer sequence Amplicon size (bp)
Universal All All F - 5′-ACTCCTACGGGAGGCAGCAGT-3′ R - 5′-GTATTACCGCGGCTGCTGGCAC-3’ 175–199
Firmicutes All All F - 5’-TGAAACTCAAAGGAATTGACG-3′ R - 5′-ACCATGCACCACCTGTC-3’ 157
Firmicutes Clostridia Cluster XIVa F - 5’-AAATGACGGTACCTGACTAA-3′ R - 5′-CTTTGAGTTTCATTCTTGCGAA-3’ 438
Firmicutes Lactobacillus spp. F - 5’-AGCAGTAGGGAATCTTCCA-3′ R - 5′-CACCGCTACACATGGAG-3’ 341
Bacteroidetes Bacteroides/Prevotella spp. F - 5’-GAGAGGAAGGTCCCCCAC-3′ R - 5′-CGCTACTTGGCTGGTTCAG-3’ 106
Actinobacteria Bifidobacterium spp. F - 5’-CGCGTCYGGTGTGAAAG-3′ R - 5′-CCCCACATCCAGCATCCA-3’ 244
Proteobacteria Enterobacter-iaceae group F - 5’-CATTGACGTTACCCGCAGAAGAAGC-3′ R - 5′-CTCTACGAGACTCAAGCTTGC-3’ 195