Fig. 2
Performance of the CPV-2 real-time RPA. a Fluorescence development over time using a dilution range of 105–100 copies of the CPV-2 standard DNA. b Semi-logarithmic regression of the data collected from eight CPV-2 real-time RPA test runs on the standard DNA using Prism Software 5.0. Run times of the real-time RPA were 4–12 min to detect 105 and 101 copies, respectively. c Probit regression analysis using SPSS software on data from eight runs. The limit of detection of 95% probability (11 copies) is indicated by a rhomboid