Fig. 3

Quantification of HfIL-1β in the plasma of non-infected house finches using the developed ELISA system. House finch plasma from randomly-selected non-infected birds were serially diluted with PBS as follows: 1:10, 1:20, 1:40, 1:80, and 1:160 (a). The diluted samples were coated with the dehydration method (60°C for 2 h), followed by sequential incubation with anti-ChIL-1β antibody (1:1000) as the primary antibody and the secondary antibody goat anti-rabbit antibody (1:2000). The HRP signal was developed with TMB solution for 45 min. The concentration of HfIL-1β plasma levels was calculated using Excel (Microsoft Corp) (b). The values represent the mean of triplicate wells with standard deviation bars. The dashed line indicates the threshold line, representing the value of negative control and limitation of the developed ELISA system (OD450 = 0.034)