Fig. 3

Immunofluorescence of canine pancreas by region. Pancreatic sections were triple-stained with fluorescent antibodies for insulin (green), glucagon (red), and somatostatin (blue) to identify variations in the clustering patterns of pancreatic cell types within different regions of the pancreas. Interestingly, glucagon-positive cells were rarely observed in the head of the pancreas, and were not associated with insulin- and somatostatin-positive cells. Insulin- and somatostatin-positive cells were observed within single clusters in the head region, which were typically comprised of only a few cells. In contrast, the tail and body region contained structures consisting of large numbers of cells that predominantly contained all three endocrine cell types, with the highest concentration and largest clusters of cells located in the tail region. Scale bars = 100 μm