Fig. 2

Representative fluorescence in situ hybridization images of colon from Experiment 1 mice. Brachyspira cells are observed with strong positive signal (red serpentine-shaped cells), whereas non-specific cellular fluorescence appears green/yellow. a Large numbers of spirochaetes are observed within the luminal mucus of “B. hampsonii” infected mouse (#2) with no lesions fed RMH diet; b Large numbers of spirochaetes are observed within the colonic crypts of a B. hyodysenteriae infected mouse (#19) fed RMH diet with catarrhal inflammation in colon. c & d) Spirochaetes in close proximity with colonic epithelium and goblet cells from a B. hyodysenteriae infected mouse (#16) fed TD85420 diet with catarrhal inflammation in colon