Fig. 4

Two forms of porcine QSOX2 can be purified via immunoprecipitation (IP). a A total protein precipitation carried out by different percentages (10–100%) of ammonium sulfate indicated that boar QSOX2 is mostly precipitated with 10%–40% ammonium sulfate. b SDS page stained with Coomassie blue indicated that anti-QSOX2 antibody could recognize and precipitate both long (marked with 2 arrowheads) and short (marked with 1 arrowheads) form of porcine QSOX2. After subtracting band intensity detected in control lanes (skeletal muscle, lane 4), we detected a 2.2 to 2.4-fold increase in band intensity at 50 kDa and a 1.3 to 1.6-fold increase at 75 kDa. c Western-Blotting analysis further confirmed the presence of both 50kD and 75kD QSOX2 in the eluted fraction. However, the majority of the long form QSOX2 remained on beads