Comparison of different NAT assays for the detection of microorganisms belonging to the class Mollicutes

BMC Veterinary Research

Table 2 Results of six PCR assays comparison with non-mollicutes bacteria

Non-mollicutes species	Strain	PCR assays^a						Real-time PCR^b melting Temperature (°C)
		1	2	3	4	5	6
Acinetobacter baumannii ^c	2208	−	+	−	−	−	+	82
Acinetobacter pleuropneumoniae ^c	M62	+	+	−	−	+	±	82.5
Aeromonas spp.^c	−	+	+	+	−	−	±	82.5
Bacillus cereus ^d	ATCC 11778	+	+	+	−	−	±	82.5
Clostridium spp. ^c	−	−	+	−	−	−	±	82
Erysipelothrix rhusiopathiae ^c	a P15	+	+	+	−	−	+	82
Escherichia coli ^d	ATCC 25922	−	−	−	−	−	±	82
Fusobacterium spp. ^c	−	+	+	+	+	+	±	82
Lactococcus garvieae ^c	4976	+	+	+	−	−	+	82.5
Lactococcus garvieae ^c	8831	+	+	+	−	−	+	82.5
Mycobacterium avium subsp. avium ^e	CHICKEN AHT1	+	+	+	−	−	+	82.5
Mycobacterium avium subsp. paratuberculosis ^e	JC31	±	+	+	−	−	+	83
Photobacterium damselae subsp. piscicida ^c	94/99	+	+	+	−	+	±	82.5
Photobacterium damselae subsp. piscicida ^c	C2	+	+	+	−	+	±	82.5
Photobacterium damselae subsp. piscicida ^c	pp3	+	+	+	−	+	±	82.5
Pseudomonas aeruginosa ^d	ATCC 27853	+	+	+	−	−	+	82
Salmonella enterica sv enteridis ^d	ATCC 13076	+	−	−	−	−	±	82.5
Staphylococcus aureus ^d	ATCC 33591	+	+	+	−	−	±	82
Streptococcus agalactiae ^d	ATCC 12386	+	+	+	−	+	+	82.5
Streptococcus suis ^e	735	+	+	+	−	+	+	82.5
Vibrio anguillarum ^c	Baumann 114	−	+	+	−	+	±	82.5

^aPCR assays: 1, Hotzel et al. [17]; 2, Spergser et al. [27], 3, McAuliffe et al. [18]; 4, Van Kuppeveld et al. [6]; 5, Botes et al. [28]; 6, Ramírez et al. [16] and real-time PCR application of PCR assays n°4 and n°5
^bReal-time PCR was performed with assays number 4 and 5, giving both the same result
^cDNA extracted, identified and donated by Infectious Diseases and Epidemiology unit (Universidad de Las Palmas de Gran Canaria, Spain)
^dFrom commercial isolates (Oxoid)
^eDNA extracted, identified and donated by Animal and Plant Health Agency (APHA, UK)
+: amplification test positive -: amplification test negative ±: amplification of more than one DNA fragment

ISSN: 1746-6148