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Fig. 3 | BMC Veterinary Research

Fig. 3

From: Geospatial and temporal associations of Getah virus circulation among pigs and horses around the perimeter of outbreaks in Japanese racehorses in 2014 and 2015

Fig. 3

Detection of viral RNA by RT-PCR for Getah virus from the supernatant of Vero cells inoculated with a pig serum. After virus isolation in Vero cells, one serum collected on 28 September 2015 in Tsuchiura city (Ibaraki Pref.) showed cytopathic effects; the nucleic acid was extracted from the culture supernatant, and RT-PCR for the nsP1 gene was performed. M, marker; lane 1, supernatant of the cytopathic effects-positive culture; lane 2, negative control; lane 3, positive control

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