Fig. 3

Detection of viral RNA by RT-PCR for Getah virus from the supernatant of Vero cells inoculated with a pig serum. After virus isolation in Vero cells, one serum collected on 28 September 2015 in Tsuchiura city (Ibaraki Pref.) showed cytopathic effects; the nucleic acid was extracted from the culture supernatant, and RT-PCR for the nsP1 gene was performed. M, marker; lane 1, supernatant of the cytopathic effects-positive culture; lane 2, negative control; lane 3, positive control