Fig. 3

Localization of prokaryotic protein expression. Images showed BAEC with P. multocida B:2 GDH7 pSRGM at 3 h post-invasion. Cells were viewed under 60× objective lens with a Leica confocal laser scanning microscope (CLSM). Images were taken of the same field under three different light paths; (I): Image taken under DIC (white) light. (II): Image taken under filtered fluorescent light (Exc. = 415 nm, Ems. = 493 nm) (blue) indicating the plasma membrane of BAEC. (III): Image taken under filtered fluorescent light (Exc. = 570 nm, Ems. = 700 nm) (red) which are referring to P. multocida B:2 GDH7. (IV): Overlay of image (II) and (III)