Table 1 Oligonucleotide primers and probes used in RT-PCR

M gene (Type A AIV) (TaqMan Real time RT-PCR)

M + 25P F

5′-AGA TGA GTC TTC TAA CCG AGG TCG-3′

100 bp

[20]

M-124 R

5′-TGC AAA AAC ATC TTC AAG TCT CTG-3′

M + 64 Probe

[FAM] 5′-TCA GGC CCC CTC AAA GCC GA-3′ [BHQ1]

HA gene (H5 AIV)

H5–155 F

5′-ACA CAT GCY CAR GAC ATA CT-3’

545 bp

[21]

H5-699R

5′-CTY TGR TTY AGT GTT GAT GT-3′

HA gene, full-length (All Type A AIV)

Bm-HA-1F

5′-TATTCGTCTCAGGGAGCAAAAGCAGGGG-3′

1778 + 29 bp

[22]

Bm-NS-890 (HA) F

5′-ATA TCG TCT CGT ATT AGT AGA AAC AAG GGT GTT TT-3′

HA gene, partial (All Type A AIV)

HA-1134F

5′-GGR ATG RTH GAY GGN TGG TAY GG-3′

640 bp

[23]

Bm-NS-890 (HA) R

5′-ATA TCG TCT CGT ATT AGT AGA AAC AAG GGT GTT TT-3′

[22]

NA gene, full-length (All Type A AIV)

Ba-NA-1F

5′-TAT TGG TCT CAG GGA GCA AAA GCA GGA GT-3’

1413 + 29 bp

[22]

Ba-NA-1413R

5′-ATATGGTCTCGTATTAGTAGAAACAAGGAGTTTTTT-3′