Fig. 1

Number of reads (classified as bacterial) within post-mortem lung and mediastinal lymph node tissues corresponding to each sample type; calves which died from the BRD (n = 38), and healthy Holstein-Friesian calves (with (n = 12) and without (n = 8) lung lesions). Fatal BRD - RVL = samples from calves with the bovine respiratory disease complex collected at regional veterinary laboratories. Healthy – lesions present = samples from healthy Holstein-Friesian calves with lung lesions. Healthy – no lesions = samples from healthy Holstein-Friesian calves without lung lesions. Boxplot = Tukey boxplot (lowest datum (whisker) is within a 1.5 interquartile range of the lower quartile, highest datum (whisker) is within a 1.5 interquartile range of the upper quartile, outliers (individual points) fall above and below these whiskers)