Table 1 Bacterial strains and plasmids used in this study
Strain/plasmid | Description | Reference/source |
|---|---|---|
Bacterial strains | ||
E. coli | ||
 BL21(DE3)pLysS | F− ompT hsdSB (rB− mB−) dcm galλ(DE3) pLysS Cmr | Promega |
 JOL232 | F− λ− ϕ80 Δ(lacZYA-argF) endA1 recA1 hadR17 deoR thi-1 glnV44 gyrA96 relA1 ΔasdA4 | Lab stock |
 JOL606 | Wild-type LT+, K99+, F6+,F18+, stx 2 +, stx 2e + STEC isolate from pig | Lab stock |
 JOL654 | Wild-type LT+,F18+,STa+, stx 2 +, stx 2e + STEC isolate from pig | Lab stock |
S. Typhimurium | ||
 JOL912 | Δlon ΔcpxR Δasd, a derivative of S. Typhimurium | [20] |
 JOL 1400 | JOL912 harboring pJHL184 | This study |
 JOL 1454 | JOL912 harboring pJHL184-stx 2eB | This study |
Plasmids | ||
 pET28a | IPTG-inducible expression vector; Kmr | Novagen |
 pET28a-stx 2eB | pET28a derivative containing stx 2eB | This study |
 pJHL184 | asd + vector, pBR ori plasmid carrying ss ompA/His6, multiple cloning site, cI857/λPR promoter, araC ParaBAD, phiX174 lysis gene E | [47] |
 pJHL184-stx 2eB | pJHL184 harboring stx 2eB gene | This study |