Fig. 2

Distribution of SNT titers of sera against BVDV-1a, BVDV-1h and BDV. a The box plots of titers of positive sera were created with the statistical software NCSS 9. Only positive titers (> 8) were included (n_BVDV-1a = 1,400; n_BVDV-1h = 1,496; n_BDV = 1,292). For titers with a value of ≤ 14 (20-fold pre-dilution), ≤ 28 (40-fold pre-dilution) and ≥ 1,810, the values 14, 28 and 1,810 were taken for calculation, respectively. The SNT titers are represented in the y-axis as logarithm to the base 2 including the standard 5-fold pre-dilution, i.e., multiplication with 5 yields the final titer. b The scatter plot of the sera samples (n = 1,555) based on their titers against BDSwiss-a, BVDV-1a and BVDV-1h was created with the statistical software NCSS 9. The black dotted line represents the threefold rotational axis on which each of the three titers per sample would have the same value and, therefore, no assignment to “BVD” or “BD” is possible (yellow). BDV-specific sera with low reaction against BVDV-1a are located in the upper left section of the cube (red), whereas BVDV-specific sera with high reaction against BVDV-1h are located in the lower right section of the cube (green). Negative sera are in the lower left corner (purple)