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Table 2 Intra- and inter-assay reproducibility for the detection of C. fetus

From: A novel real-time PCR assay for quantitative detection of Campylobacter fetus based on ribosomal sequences

Genome copies/reaction Intra-assay variations Inter-assay variations
  Mean Ct (from – to) CV (from – to) Mean Ct CV
1 × 101 -a - - -
1 × 102 36.57–37.69 0.97–2.1 37.13 2.19
1 × 103 33.68–34.11 0.48–1.15 33.89 1.05
1 × 104 30–30.07 0.25–0.16 30.03 0.23
1 × 105 26.37–26.46 0.14–0.27 26.41 0.26
1 × 106 22.62–22.86 0.18–0.7 22.74 0.73
1 × 107 19.02–19.23 0.5–0.83 19.12 0.86
  1. CV coefficient of variation of Ct values [%]
  2. aCt value out of dynamic range