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Table 2 Intra- and inter-assay reproducibility for the detection of C. fetus

From: A novel real-time PCR assay for quantitative detection of Campylobacter fetus based on ribosomal sequences

Genome copies/reaction

Intra-assay variations

Inter-assay variations

 

Mean Ct (from – to)

CV (from – to)

Mean Ct

CV

1 × 101

-a

-

-

-

1 × 102

36.57–37.69

0.97–2.1

37.13

2.19

1 × 103

33.68–34.11

0.48–1.15

33.89

1.05

1 × 104

30–30.07

0.25–0.16

30.03

0.23

1 × 105

26.37–26.46

0.14–0.27

26.41

0.26

1 × 106

22.62–22.86

0.18–0.7

22.74

0.73

1 × 107

19.02–19.23

0.5–0.83

19.12

0.86

  1. CV coefficient of variation of Ct values [%]
  2. aCt value out of dynamic range