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Table 2 List of analytical methods

From: Biochemical and proteomic analyses of the physiological response induced by individual housing in gilts provide new potential stress markers

Biochemical parameter

Sample type

Method

Manufacturer

NEFAs

Serum

NEFA-C reagent

Wako Chemicals GmbH (Neuss, Germany)

Cholesterol

Serum

CHOP-PAP-method

Olympus System Reagents (OSR) -Olympus Diagnostica GmbH

Triglycerides

Serum

GPO-PAP method

OSR

Urea

Serum

GLDH method

OSR

Creatinine

Serum

Jaffé method

OSR

Total protein

Serum

Biuret method

OSR

Creatine kinase (CK)

Serum

IFCC method

OSR

Alanine aminotransferase (ALT)

Serum

IFCC method, without pyridoxal phospate

OSR

Alkaline phosphatase (ALP)

Serum

IFCC method

OSR

Haptoglobin

Serum

Phase Haptoglobin kit (colorimetric assay

Tridelta Ltd (County Kildare, Ireland)

C-reactive protein (CRP)a

Serum

Immunoturbidimetric method

OSR (#6147)

Pig-MAP

Serum

ELISA

PigChamp ProEuropa (Segovia, Spain)

Cortisol

Serum

ELISA

DRG Diagnostics (Marburg, Germany)

Cortisol

Saliva

ELISA

DRG Diagnostics

Glutathione peroxidase (GPx)

Erythrocyte lysate

Cumene Hydroperoxide (Ransel)

Randox Laboratories Ltd

Superoxide dismutase (SOD)

Erythrocyte lysate

Xanthine oxidase (Ransod)

Randox Laboratories Ltd

Total glutathione (tGSH)

Deproteinized blood

Glutathione Assay

Northwest (Vancouver, WA)

  1. aValidated for porcine samples [42]