Fig. 2

Antimicrobial activity of chicken HDPs against E.coli. E. coli (6 × 10⁶) were incubated with indicated amounts of peptide for 2 h and cell viability was compared to untreated cells. Nineteen chicken HDPs were tested under 5 μM concentrations (a) and 12 HDPs that showed more than 90 % killing activity in 5 μM treatment were selected for assay at lower concentrations (0.5, 1, 2.5, 5 μM) (b). Each bar represents the mean ± S.D. value of at least three independent experiments. Non-treated cell considered as 100 % cell viability and peptides treated cell viability was compared and calculated in percentile. *** indicate p ≤ 0.001, ** indicate p ≤ 0.01 and * indicate p ≤ 0.05