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Table 2 Details of the enzyme immunoassays (EIAs) used

From: Non-invasive assessment of adrenocortical activity as a measure of stress in giraffe (Giraffa camelopardalis)

EIA (source laboratory, abbrev.)

Sensitivity (ng/g feces)

Intra-assay CVa

Inter-assay CVb

References

Cortisol (Munro, R4866)

2.3

4.9 %

3.7 %, n = 4

[77, 78]

Corticosterone (Munro, CJM06)

17.6

8.1 %

8.1 %, n = 4

[79, 80]

11-oxoetiocholanolone I (Palme, 72a, 11,17-DOA)

7.2

4.8 %

12.3 %, n = 5

[63, 81]

11-oxoetiocholanolone II (Palme, 72T, 3α,11-oxo-CM)

9.6

8.8 %

13.8 %, n = 4

[61, 67]

5α-pregnane-3β,11β,21-triol-20-one (Palme, 37e, 3β,11β-diol-CM)

9.6

6.1 %

9.5 %, n = 4

[34]

11ß-hydroxyetiocholanolone (Palme, 69a, 3α,11β-diol-CM)

48.0

9.1 %

9.7 %, n = 4

[54, 82]

  1. aIntra-assay CV = SD/mean percent binding for 10 wells each of high- and low-concentrated pool samples, averaged across concentrations
  2. bInter-assay CV = SD/mean percent binding for standards (72T, 69a) or high- and low-concentrated pool samples (other assays), averaged across concentrations, n indicates number of plates used
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BMC Veterinary Research

ISSN: 1746-6148

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