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Table 2 Details of the enzyme immunoassays (EIAs) used

From: Non-invasive assessment of adrenocortical activity as a measure of stress in giraffe (Giraffa camelopardalis)

EIA (source laboratory, abbrev.) Sensitivity (ng/g feces) Intra-assay CVa Inter-assay CVb References
Cortisol (Munro, R4866) 2.3 4.9 % 3.7 %, n = 4 [77, 78]
Corticosterone (Munro, CJM06) 17.6 8.1 % 8.1 %, n = 4 [79, 80]
11-oxoetiocholanolone I (Palme, 72a, 11,17-DOA) 7.2 4.8 % 12.3 %, n = 5 [63, 81]
11-oxoetiocholanolone II (Palme, 72T, 3α,11-oxo-CM) 9.6 8.8 % 13.8 %, n = 4 [61, 67]
5α-pregnane-3β,11β,21-triol-20-one (Palme, 37e, 3β,11β-diol-CM) 9.6 6.1 % 9.5 %, n = 4 [34]
11ß-hydroxyetiocholanolone (Palme, 69a, 3α,11β-diol-CM) 48.0 9.1 % 9.7 %, n = 4 [54, 82]
  1. aIntra-assay CV = SD/mean percent binding for 10 wells each of high- and low-concentrated pool samples, averaged across concentrations
  2. bInter-assay CV = SD/mean percent binding for standards (72T, 69a) or high- and low-concentrated pool samples (other assays), averaged across concentrations, n indicates number of plates used