Fig. 6
From: Identification and characterization of two CD4 alleles in Microminipigs
Expression of the CD4-FLAG vectors in HeLa cells. We expressed only the constructs CD4-FLAG with complete sequences in HeLa cells as described in Fig. 5. a Cells stained with rabbit anti-FLAG antibody followed by Alexa Fluor 488 goat anti-rabbit IgG antibody. b Cells stained with PE conjugated mouse anti-pig CD4 antibody. c Cells stained with the overlay of three fluorophore signals; anti-FLAG antibody, anti-pig CD4 antibody, and Hoechst 33342