Pigs immunized with a novel E2 subunit vaccine are protected from subgenotype heterologous classical swine fever virus challenge

Table 2 CSFV were cleared from the nasal cavity in pigs vaccinated with E2 subunit vaccine 15 days post challenge

Nasal swab		3 DPC		6 DPC		9 DPC		12 DPC		15 DPC
Treatment	Pig #	Ct value	TCID₅₀	Ct value	TCID₅₀	Ct value	TCID₅₀	Ct value	TCID₅₀	Ct value	TCID₅₀
(−/−)	51	ND	ND	ND	ND	ND	ND	ND	ND	(−)	(−)
(−/−)	52	ND	ND	ND	ND	ND	ND	(−)	(−)	(−)	(−)
(−/−)	53	(−)	(−)	(−)	(−)	(−)	(−)	(−)	(−)	(−)	(−)
(−/−)	54	(−)	(−)	(−)	(−)	39	37	(−)	(−)	(−)	(−)
(−/−)	55	(−)	(−)	34	184	34	560	(−)	(−)	(−)	(−)
(−/+)	56	(−)	(−)	35	95	33	1521	28	19,033	33	2867
(−/+)	57	(−)	(−)	32	876	24	300,220	20	1,882,476	22	904,988
(−/+)	58	37	31	34	293	24	411,326	21	1,438,113	21	1,502,256
(−/+)	59	(−)	(−)	32	1065	21	3,182,081	pig died
(−/+)	60	38	19	34	261	25	293,326	21	1,451,296	22	745,304
One-dose	61	(−)	(−)	35	124	36	166	36	106	(−)	(−)
One-dose	62	(−)	(−)	(−)	(−)	38	52	37	42	36	109
One-dose	63	(−)	(−)	37	35	37	83	33	495	(−)	(−)
One-dose	64	(−)	(−)	34	224	(−)	(−)	37	68	(−)	(−)
One-dose	65	(−)	(−)	36	68	37	78	34	301	(−)	(−)
Two-dose	66	(−)	(−)	37	38	39	28	35	145	(−)	(−)
Two-dose	67	(−)	(−)	36	60	38	67	35	221	(−)	(−)
Two-dose	68	(−)	(−)	(−)	(−)	34	2398	34	283	(−)	(−)
Two-dose	69	(−)	(−)	36	56	37	79	34	269	37	42
Two-dose	70	(−)	(−)	36	58	(−)	(−)	34	330	(−)	(−)

ND not done; (−): Undetectable; Pigs were challenged with CSFV 35 days post first vaccination. CSFV RNA in the blood was measured by real-time RT-PCR as described in Methods section
(−/−): Control pigs without CSFV challenge; (−/+): Control pigs challenged with CSFV
One-dose: Pigs vaccinated with one dose KNB-E2 and then challenged with CSFV
Two-dose: Pigs vaccinated with KNB-E2 twice and then challenged with CSFV

ISSN: 1746-6148