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Fig. 1 | BMC Veterinary Research

Fig. 1

From: Serological response and protection level evaluation in chickens exposed to grains coated with I2 Newcastle disease virus for effective oral vaccination of village chickens

Fig. 1

Study design of the experimental NDI2 vaccine trial in Bovans brown chickens. Vaccine carrier groups (experimental units) were listed from No. 1 -15 in this figure. Sera collection was performed across age in days (indicator, blue color) to get 1ml sera per chick at each sampling day from wing vein until day 119. However, for chickens of barely and cracked maize group the last sampling was made on day 126, not on day 119. Vaccination was given at a rate of 107 EID50 NDV I 2 per dose per chick three times (indicator, black color). The value of vaccination by each grain carrier was measured by monitoring antibody production post vaccination in the regularly collected sera and by survival rate of the chickens post artificial inoculation of a virulent local Alemaya strain ND virus at a rate of 0.5 × 106.5 EID50 per chick of all experimental units intramuscularly at breast muscle at day129 (indicator, red color)

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