Validation of an automated assay for the measurement of cupric reducing antioxidant capacity in serum of dogs

BMC Veterinary Research

Table 1 Mean, standard deviation (SD), intra- and inter-assay CVs variation in TAC_c concentrations of canine serum samples (two with lower concentration and two with high concentrations) measured in the two apparatus

Apparatus	Validation parameter	Number of samples	Mean (mmol Trolox equiv./L)	SD	CV (%)
Cobas Mira Plus	Intra-assay	2	0.2325	0.004	1.9
	Intra-assay	2	0.3823	0.007	1.8
	Inter-assay	2	0.2254	0.015	6.8
	Inter-assay	2	0.3766	0.019	5.1
Olympus AU400	Intra-assay	2	0.2623	0.003	1.2
	Intra-assay	2	0.3999	0.003	0.7
	Inter-assay	2	0.2533	0.022	8.6
	Inter-assay	2	0.3842	0.030	7.8

TAC _c total antioxidant capacity using the cupric reducing antioxidant capacity methodology and the bathocuproinedisulfonic acid disodium salt, SD standard deviation, CV coefficient of variation

ISSN: 1746-6148