Table 3 Relative amount of TRPM1 mRNA detected in cultured choroidal melanocytes transfected with expression vectors coding for MITF-M and/or Nova-1 expression. n = 6 replicate rt q-pcr reactions
Effect of transfection on relative TRPM1 mRNA level | |||||
|---|---|---|---|---|---|
Wild type melanocytes | LP/LP melanocytes | P | |||
ΔΔCT | Fold change | ΔΔCT | Fold change | ||
MITF-M | −2.1 ± 0.8 | 4.3 | −0.8 ± 1.9 | 1.74 | 0.14 |
Nova-1 | 2.3 ± 0.8 | 0.20 | 5.8 ± 1.1 | 0.018 | <0.001 |
MITF-M & Nova-1 | 3.8 ± 0.3 | 0.072 | 7.2 ± 0.9 | 0.0068 | <0.001 |