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Table 3 Relative amount of TRPM1 mRNA detected in cultured choroidal melanocytes transfected with expression vectors coding for MITF-M and/or Nova-1 expression. n = 6 replicate rt q-pcr reactions

From: Redundant contribution of a Transient Receptor Potential cation channel Member 1 exon 11 single nucleotide polymorphism to equine congenital stationary night blindness

  Effect of transfection on relative TRPM1 mRNA level
Wild type melanocytes LP/LP melanocytes P
ΔΔCT Fold change ΔΔCT Fold change  
MITF-M −2.1 ± 0.8 4.3 −0.8 ± 1.9 1.74 0.14
Nova-1 2.3 ± 0.8 0.20 5.8 ± 1.1 0.018 <0.001
MITF-M & Nova-1 3.8 ± 0.3 0.072 7.2 ± 0.9 0.0068 <0.001