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Table 1 PCR primers used to amplify human (H s) and equine (E c) cDNA templates

From: Redundant contribution of a Transient Receptor Potential cation channel Member 1 exon 11 single nucleotide polymorphism to equine congenital stationary night blindness

Primer 5′ – 3′ sequence Product bp Efficiency
E c Nova-1 F16 CAA ACT ACC ACC AAG TCC TC   
E c Nova-1 R224 CTC ACA GTG ACA ACC CTC TC 209 110 %
E c Nova-1 F1024 GGG ACA TTT GCA TTA GGT AG   
E c Nova-1 R1454 ATG GGG TAA AGG AGG GGT TA 431  
H s Nova-1 F310 ACA TTG CCA TCT TCC CCA AC   
H s Nova 1 R371 TGG AGG TGG TCA TGG GAT CA 62  
E c MITF-M F 408 CTT GAT GGA TCC TGC CTT G   
E c MITF-M R573 GGG AAA AAT ACA CGC TGT GA 166 110 %
E c βactin F55 GCC GTC TTC CCC TCC AT   
E c βactin R135 GCC C AC GTA TGA GTC CTT CTG 81 121 %
E c TRPM1 F2254 GAC GAC ATC TCC CAG GAT CT   
E c TRPM1 R2326 TGC TCG TCG TGC TTA TAG GA 73 113 %